Coding

Part:BBa_K4200780:Design

Designed by: Rahil Ummar Faruk Abbu, Tushar Mishra   Group: iGEM22_MIT_MAHE   (2022-10-11)


Ssp GyrB S11 split intein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The intein sequence was codon optimized through Benchling. The internal restriction sites with respect to NdeI and XhoI were removed.

Source

Synechocystis sp. PCC6803 genomic DNA, synthetic

References

G. Volkmann and X.-Q. Liu, “Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity,” FEBS Journal, vol. 278, no. 18, pp. 3431–3446, 2011.

J. Appleby, K. Zhou, G. Volkmann and X. Liu, "Novel Split Intein for trans-Splicing Synthetic Peptide onto C Terminus of Protein", Journal of Biological Chemistry, vol. 284, no. 10, pp. 6194-6199, 2009.