Coding
Part:BBa_K4200780:Design
Designed by: Rahil Ummar Faruk Abbu, Tushar Mishra Group: iGEM22_MIT_MAHE (2022-10-11)
Ssp GyrB S11 split intein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The intein sequence was codon optimized through Benchling. The internal restriction sites with respect to NdeI and XhoI were removed.
Source
Synechocystis sp. PCC6803 genomic DNA, synthetic
References
G. Volkmann and X.-Q. Liu, “Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity,” FEBS Journal, vol. 278, no. 18, pp. 3431–3446, 2011.
J. Appleby, K. Zhou, G. Volkmann and X. Liu, "Novel Split Intein for trans-Splicing Synthetic Peptide onto C Terminus of Protein", Journal of Biological Chemistry, vol. 284, no. 10, pp. 6194-6199, 2009.